Identifying the Targets of the Amplifying Pathway for Insulin Secretion in Pancreatic β-Cells by Kinetic Modeling of Granule Exocytosis

Abstract

A kinetic model for insulin secretion in pancreatic β-cells is adapted from a model for fast exocytosis in chromaffin cells. The fusion of primed granules with the plasma membrane is assumed to occur only in the “microdomain” near voltage-sensitive L-type Ca 2+-channels, where [Ca 2+] can reach micromolar levels. In contrast, resupply and priming of granules are assumed to depend on the cytosolic [Ca 2+]. Adding a two-compartment model to handle the temporal distribution of Ca 2+ between the microdomain and the cytosol, we obtain a unified model that can generate both the fast granule fusion and the slow insulin secretion found experimentally in response to a step of membrane potential. The model can simulate the potentiation induced in islets by preincubation with glucose and the reduction in second-phase insulin secretion induced by blocking R-type Ca 2+-channels (Ca V2.3). The model indicates that increased second-phase insulin secretion induced by the amplifying signal is controlled by the “resupply” step of the exocytosis cascade. In contrast, enhancement of priming is a good candidate for amplification of first-phase secretion by glucose, cyclic adenosine 3′:5′-cyclic monophosphate, and protein kinase C. Finally, insulin secretion is enhanced when the amplifying signal oscillates in phase with the triggering Ca 2+-signal.