Identifying CEACAM1 as a potential prognostic biomarker for basal-like breast cancer by bioinformatics analysis and in vitro experiments.

Abstract

Background: Carcinoembryonic antigen related cell adhesion molecule-1 (CEACAM1) is a very important intercellular adhesion molecule, and its prognostic relevance to breast cancer (BC), especially basal-like breast cancer (BLBC), remains poorly understood. Methods: CEACAM1 mRNA expression data for BC were sourced from the Cancer Genome Atlas (TCGA) database. Kaplan-Meier survival analysis and Cox regression analysis were used to evaluate the prognostic relationship between CEACAM1 expression and BC. Signaling pathways associated with CEACAM1 were analysed using Gene Set Enrichment Analysis (GSEA) and Kyoto Encyclopedia of Genes and Genomes (KEGG) analysis. Moreover, cell counting kit-8 (CCK-8), flow cytometry, transwell and wound-healing assays were employed to identify the biological functions of CEACAM1 in BLBC. Results: CEACAM1 was correlated with overall survival (OS) of BLBC patients. Compared with the subgroup with better prognosis, the levels of CEACAM1 mRNA expression were significantly lower in the subgroup of BLBC with poorer prognosis. Both univariate and multivariate Cox regression analysis suggested that down-regulation of CEACAM1 expression may be an independent factor for poor prognosis in BLBC patients. GSEA and KEGG analysis revealed that CEACAM1 was negatively related with signaling pathways including extracellular matrix (ECM) receptor interaction, focal adhesion, and cell adhesion. The results of in vitro experiments indicated that CEACAM1 not only induced apoptosis of BLBC cells, but also inhibited the invasive and metastatic ability of cancer cells. Conclusions: CEACAM1 may contribute to improving the OS of BLBC patients due to its ability to inhibit the proliferation and metastasis of cancer cells. Therefore, CEACAM1 could be used as a potential prognostic biomarker and therapeutic target in BLBC.