Abstract
BackgroundMicroRNAs (miRNAs) and short interfering RNAs (siRNAs) are two major classes of small RNAs. They play important regulatory roles in plants and animals by regulating transcription, stability and/or translation of target genes in a sequence-complementary dependent manner. Over 4,000 miRNAs and several classes of siRNAs have been identified in plants, but in tobacco only computational prediction has been performed and no tobacco-specific miRNA has been experimentally identified. Wounding is believed to induce defensive response in tobacco, but the mechanism responsible for this response is yet to be uncovered.ResultsTo get insight into the role of small RNAs in damage-induced responses, we sequenced and analysed small RNA populations in roots and leaves from wounding or topping treated tobacco plants. In addition to confirmation of expression of 27 known miRNA families, we identified 59 novel tobacco-specific miRNA members of 38 families and a large number of loci generating phased 21- or 24-nt small RNAs (including ta-siRNAs). A number of miRNAs and phased small RNAs were found to be responsive to wounding or topping treatment. Targets of small RNAs were further surveyed by degradome sequencing.ConclusionsThe expression changes of miRNAs and phased small RNAs responsive to wounding or topping and identification of defense related targets for these small RNAs suggest that the inducible defense response in tobacco might be controlled by pathways involving small RNAs.
Highlights
IntroductionMicroRNAs (miRNAs) and short interfering RNAs (siRNAs) are two major classes of small RNAs. MicroRNAs (miRNAs) and short interfering RNAs (siRNAs) are two major classes of small RNAs
MicroRNAs and short interfering RNAs are two major classes of small RNAs
Small RNAs are a group of regulatory molecules that fall into two major classes, microRNAs and short interfering RNAs
Summary
MicroRNAs (miRNAs) and short interfering RNAs (siRNAs) are two major classes of small RNAs. MicroRNAs (miRNAs) and short interfering RNAs (siRNAs) are two major classes of small RNAs They play important regulatory roles in plants and animals by regulating transcription, stability and/or translation of target genes in a sequence-complementary dependent manner. Small RNAs are a group of regulatory molecules that fall into two major classes, microRNAs (miRNAs) and short interfering RNAs (siRNAs). They play important roles in biological systems in eukaryotes by suppressing expression of target genes at the transcriptional and/or post-transcriptional level through specific base pairing with their targets [1]. A number of studies have been performed to reveal the biogenesis of miRNAs and the [16,17,18]; miR159 has been shown to be required for normal anther development by mediating the expression of GAMYB-related genes [19]
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