Abstract
Transcriptional changes accompany induced resistance of plants against insect feeding, and monitoring transcriptional reorganization triggered in response to herbivory is an essential step for deciphering the molecular basis of the resistance. To isolate up-regulated genes in tea leaves under mild infestation of green leafhopper ( Empoasca vitis Göthe), a subtractive cDNA library was constructed using the suppression subtractive hybridization strategy. Subsequent differential screening, DNA sequencing analyses, and real-time PCR analyses identified 21 unique genes up-regulated by leafhopper feeding from the library. Among the genes likely to be directly involved in defense, those encoding pathogen-related protein and type 2 ribosome-inactivating protein were found in the subtractive library. In addition, genes involved in the CBL (calcineurin B-like)–CIPK (CBL-interacting protein kinases) pathway, cell wall fortification, secondary metabolite synthesis, water transport, and oxidative stress protection were identified in our experiment. Besides, a number of genes that have not been previously associated with defense responses against insects were isolated.
Sign-in/Register to access full text options 
Talk to us
Join us for a 30 min session where you can share your feedback and ask us any queries you have
Schedule a callDisclaimer: All third-party content on this website/platform is and will remain the property of their respective owners and is provided on "as is" basis without any warranties, express or implied. Use of third-party content does not indicate any affiliation, sponsorship with or endorsement by them. Any references to third-party content is to identify the corresponding services and shall be considered fair use under The CopyrightLaw.
