Gene | VOL. 823
Read

Identification of thrombin as a key regulator of chondrocyte catabolic activity through RNA-Seq and experimental verification.

Publication Date May 20, 2022

Abstract

The present study was designed to explore the relationship between thrombin and catabolic activity in chondrocytes. Primary rat chondrocytes were cultured for 24h with rat serum (RS), rat plasma (RP), or rat plasma supplemented with thrombin (RPT). RNA-sequencing was then performed. Cell proliferation was analyzed by EdU uptake, CCK-8 assays and protein-protein interaction (PPI) network of proliferation-related genes. Heatmaps were used to visualize differences in gene expression. Gene Ontology (GO) enrichment analyses of up- and down-regulated differentially expressed genes were conducted. Molecular probes were used to label the endoplasmic reticulum in chondrocytes from three treatment groups. Immunofluorescence and Safranin O staining were used to assess type II collagen (Col2a1) expression and proteoglycan synthesis, whereas Lox expression was assessed by immunocytochemistry. The expression of enzymes involved in the synthesis and maturation of extracellular matrix (ECM) components and chemokines were measured by qPCR while matrix metalloproteinases (MMPs) levels were evaluated by Western blotting. Relevant nodules were selected through further PPI network analyses. A total of 727 and 1162 genes were up- and down-regulated based on the Venn diagrams comparison among groups. Thrombin was thus able to promote chondrocyte proliferation and a shift towards fibrotic morphology, while upregulating MMPs and chemokines linked to ECM degradation. In addition, thrombin decreased the enzyme expression involved in the synthesis and ...

Concepts
Powered ByUnsilo

Endoplasmic Reticulum In Chondrocytes
Maturation Of Extracellular Matrix
Primary Rat Chondrocytes
Synthesis Of Extracellular Matrix
Protein-protein Interaction Network Analyses
EdU Uptake
Extracellular Matrix
Activity In Chondrocytes
Matrix Metalloproteinases
Lox Expression

Introducing Weekly Round-ups!Beta

Powered by R DiscoveryR Discovery

Round-ups are the summaries of handpicked papers around trending topics published every week. These would enable you to scan through a collection of papers and decide if the paper is relevant to you before actually investing time into reading it.

Climate change Research Articles published between Jun 13, 2022 to Jun 19, 2022

R DiscoveryJun 20, 2022
R DiscoveryArticles Included:  5

Understanding the effects of the snow ratio on glacier mass balance under variable climatic conditions is crucial for predicting how glaciers will res...

Read More

Good health Research Articles published between Jun 13, 2022 to Jun 19, 2022

R DiscoveryJun 20, 2022
R DiscoveryArticles Included:  5

In West Africa, the various types of diabetes according to WHO or the American Diabetes Association (ADA) are endemic; a particular type referred to a...

Read More

Gender Equality Research Articles published between Jun 13, 2022 to Jun 19, 2022

R DiscoveryJun 20, 2022
R DiscoveryArticles Included:  5

This study aims to evaluate changes in the presence of women on Spanish boards after the Unified Good Governance Code of Listed Companies (2006) and t...

Read More

Coronavirus Pandemic

You can also read COVID related content on R COVID-19

R ProductsCOVID-19

ONE PROBLEM . ONE PURPOSE . ONE PLACE

Creating the world’s largest AI-driven & human-curated collection of research, news, expert recommendations and educational resources on COVID-19

COVID-19 Dashboard