Abstract
The initial step in transfer of glucose from mother to fetus is facilitated diffusion transport across the microvillous membrane of the placental syncytium (1). We have used 3H-cytochalasin B as a photoaffinity label to identify the transport protein involved. Two binding proteins were present, one of which is apparently the glucose transport protein and one of which is actin. The two were identified by competition labeling with D-glucose, and cytochalasin E. They were separated by selective extraction with dimethyl maleic anhydride. The glucose transport protein is apparently a single molecular species of 52,000 molecular weight.
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