Identification of the antigenic determinants of factors 8, 9, and 34 of genus Candida

Abstract

We investigated the antigenic determinants of factors 8, 9, and 34 of the genus Candida among pathogenic yeasts by enzyme-linked immunosorbent assay (ELISA) using mannans of Saccharomyces cerevisiae wild type and mutant types, mnn 1- mnn 4 and mnn 2. Results of ELISA including antisera against the antigenic factors of genus Candida (Candida Check, Iatron; FAbs) indicated that these three types of mannan distinctly react with FAbs 34, 8 and 9, respectively. To identify the recognition sites of these FAbs, we compared the ability of various oligosaccharides to inhibit the binding of the mannans to FAbs. The results indicated that FAb 34 preferentially recognizes linear side chains containing a non-reducing terminal α-1,3-linked mannose residue, Man α1 → 3Man α1 → (2Man α1 →) n 2Man ( n ≥ 0), and that one of the recognition sites of FAb 9 is linear α-1,6-linked oligomannosyl series, Man α1 → (6Man α1 →) n 6-Man ( n ≥ 2). On the other hand, the recognition site of FAb 8 apparently consisted of two α-1,2-linked oligomannosyl side chains and an α-1,6-linked mannose residue that originated from the mannan backbone, Man α1 → 2Man α1 → 2(Man α1 → 2Man α1 → 6)Man.