Abstract
Colorectal cancer (CRC) is one of the most common malignancies of the digestive system. Recent studies have revealed the importance of RNA-binding proteins (RBPs) in tumorigenesis, but their role in CRC remains unclear. The present study systematically analyzed the relationships between RBPs and CRC using data from The Cancer Genome Atlas. We detected 483 differentially expressed RBPs and identified a series of pathways and processes using GO (Gene Ontology) analysis and KEGG (Kyoto Encyclopedia of Genes and Genomes) pathway analysis. Analyzing protein–protein interactions and modules identified the edges and modules of RBPs. Univariate and multivariate Cox regression analyses were then used to construct a prognostic model that included 13 RBPs. Survival analyses indicated that the overall survival (OS) was significantly lower for CRC patients in the high-risk group than for those in the low-risk group, and that high risk scores were associated with poor OS. Finally, we constructed a nomogram that included 13 RBPs for calculating the estimated survival probabilities of CRC patients at 1, 2, and 3 years. Calibration plots indicated good conformity between the predicted and observed outcomes. This study has revealed that the expression of RBPs differs between CRC and normal tissues. A prognostic model based on 13 RBP coding genes has been developed that can provide independent prognoses of CRC.
Highlights
Colorectal cancer (CRC) is one of the most common malignancies of the digestive system [1]
As for biological processes, upregulated genes were significantly associated with ncRNA processing, ribosome biogenesis, rRNA metabolic process, rRNA processing, and RNA phosphodiester bond hydrolysis
Downregulated genes were significantly associated with cellular amide metabolic process, regulation of translation, regulation of mRNA metabolic process, RNA splicing, and negative regulation of translation
Summary
Colorectal cancer (CRC) is one of the most common malignancies of the digestive system [1]. Recent changes in diet characteristics, population aging, and living standards have resulted in gradual increases in the incidence and mortality of CRC in China [2]. RNA-binding proteins (RBPs) perform various functions to maintain cellular homeostasis. These proteins play vital roles in regulating numerous essential cellular processes, including RNA splicing, modification, transport, localization, stability, degradation, and translation [3, 4]. It has been estimated that more than 1,500 proteins can bind RNA in the human genome [5]. Any significant change or disturbance in the RBPs regulating these essential functions can lead to diseases, including cancer [4, 6]
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