Abstract
Cold stress in rice is a critical factor limiting growth and yield in temperate regions. In this study, we identified quantitative trait loci (QTL) conferring cold tolerance during the booting stage using a recombinant inbred line population derived from a cross between a cold-susceptible Tongil-type cultivar Milyang23 and a cold-tolerant japonica cultivar Giho. A phenotypic evaluation was performed in a cold-water-irrigated field (17 °C) and a temperature-controlled (17 °C/17 °C air and water) greenhouse at the booting stage. Four QTL, including two on chromosome 1 and one each on chromosomes 6 and 9, were identified in the cold-water-irrigated field, with an R2 range of 6.3%–10.6%. Three QTL, one on each of chromosomes 2, 6 and 9, were identified under the temperature-controlled greenhouse condition, with an R2 range of 5.7%–15.1%. Among these, two QTL pairs on chromosomes 6 (qSFF6 and qSFG6) and 9 (qSFF9 and qSFG9) were detected in the cold treatments of both field and greenhouse screenings. Our results provide a reliable dual-screening strategy for rice cold tolerance at the booting stage.
Highlights
Rice (Oryza sativa L.) is one of the most important staple crops worldwide and feeds almost half of the world’s population
We identified two reliable quantitative trait loci (QTL) for rice Cold tolerance at the booting stage (CTB) by examining a recombinant inbred line (RIL) population derived from a cross between Milyang23 (Tongil-type) and Giho using a dual phenotyping system with a cold-water-irrigated field condition and a temperature-controlled greenhouse condition
By evaluating major agronomic traits of the RIL population, we report a QTL for CTB that is not affected by other traits such as days to heading, culm and panicle length
Summary
Rice (Oryza sativa L.) is one of the most important staple crops worldwide and feeds almost half of the world’s population. When rice plants are exposed to cold stress, various symptoms appear, such as poor germination, slow seedling growth, delayed vegetative growth, discoloration and poor grain fertility and filling, depending on the developmental stage [1] These symptoms inhibit the normal growth of rice and lead to yield losses. Cold tolerance at the booting stage (CTB) is often evaluated based on spikelet fertility in the field using cold water irrigation at 18–19 ◦ C Using this method, large varietal differences and extensive quantitative trait loci (QTL) have been identified. A QTL for CTB, qCTB8, was identified on chromosome 8 using F2 , F3 and F7 populations derived from a cross between two temperate japonica cultivars Hokkai-PL9 and Hokkai287 [15]. We identified two reliable QTL for rice CTB by examining a RIL population derived from a cross between Milyang. By evaluating major agronomic traits of the RIL population, we report a QTL for CTB that is not affected by other traits such as days to heading, culm and panicle length
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