Abstract
Since the cost of Ophiocordyceps sinensis, an important fungal drug used in Chinese medicine, has increased dramatically, and the counterfeits may have adverse health effects, a rapid and precise marker using the peptide mass spectrometry identification system could significantly enhance the regulatory capacity. In this study, we determined the marker peptides in the digested mixtures of fungal proteins in wild O. sinensis fruiting bodies and various commercially available mycelium fermented powders using ultra-performance liquid chromatography/Orbitrap Fusion mass spectrometry coupled with chemometrics. The results indicated the following marker peptides: TLLEAIDSIEPPK (m/z 713.39) was identified in the wild O. sinensis fruiting body, AVLSDAITLVR (m/z 579.34) was detected in the fermented O. sinensis mycelium powder, FAELLEK (m/z 849.47) was found in the fermented Ophiocordyceps mycelium powder, LESVVTSFTK (m/z 555.80) was discovered in the artificial Ophiocordyceps mycelium powder, and VPSSAVLR (m/z 414.75) was observed in O. mortierella mycelium powder. In order to verify the specificity and applicability of the method, the five marker peptides were synthesized and tested on all samples. All in all, to the best of our knowledge, this is the first time that mass spectrometry has been employed to detect the marker peptides of O.sinensis and its related products.
Highlights
IntroductionChongcao (the sexual stage of the Ophiocordyceps sinensis) is an important traditional fungal drug that has been commonly used for hundreds of years as a tonic and/or drug
Chongcao is an important traditional fungal drug that has been commonly used for hundreds of years as a tonic and/or drug
The results demonstrated that this method could be used to authenticate wild O. sinensis and its related cultured Ophiocordyceps mycelia powder and the mixed commercial products
Summary
Chongcao (the sexual stage of the Ophiocordyceps sinensis) is an important traditional fungal drug that has been commonly used for hundreds of years as a tonic and/or drug. Molecules 2018, 23, 1013 effect of tonifying the kidney and replenishing lung, stanching bleeding, and resolving phlegm. It could be used to treat 21 ailments and be a potential adjuvant chemotherapeutic agent in non-small cell lung cancer, liver cancer, and breast therapy [2,3]. Despite the hazardous effects to the humanhealth, chongcao possesses manyanti-tumor and antioxidant activities, as well asthe capacity to modulate the immune system and treat fatigue, night sweating, hyposexuality, hyperglycemia, liver disease, and heart disease [4,5,6,7,8,9]. In spite of the increasing price, its manufacture and sales were strictly regulated in 2016 by the China Food and
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