Abstract
BackgroundAn in vivo animal study and a prospective clinical study have indicated that bone marrow aspirate (BMA) augments spinal arthrodesis. However, there is no quantified data to explain why fusion rate can be augmented by BMA in lumbar posterolateral fusion.MethodsTo analyze the proportion of mesenchymal stem cells (MSCs) and osteogenic factors in human BMA and peripheral blood (PB) of the same patient. Autologous BMA and PB from the patients were analyzed by flow cytometry (FACS) using cell markers for MSCs. The osteogenic potential of MSCs was determined by alkaline phosphatase (ALP) activity and calcium level quantification. Proteomics were used for the qualitative and quantitative mapping of the whole proteome from BMA and PB plasma. The mass-to-charge ratio was calculated by time-of-flight mass spectrometry (TOF-MS). The overexpression of protein was confirmed using Western blot analysis.ResultsThe proportion of MSCs (CD34−/CD29+/CD105+) was higher in the BMA than that in the PB. Colony-forming cell (CFC) assays suggested that fewer colonies were formed in PB cultures than in BMA culture. There was no significant difference in the osteogenic potential of the MSCs between the PB and BMA. Proteomic mass spectrometry assays suggested that the levels of catalase (osteoclast inhibitor) and glutathione peroxidase 3 (osteogenic biomarker) were higher in the BMA than those in the PB, and this was confirmed by Western blot analysis.ConclusionsThe proportions of MSCs and osteogenic factors were higher in the BMA than in the PB. This may explain why fusion rate can be augmented by BMA in lumbar posterolateral fusion.
Highlights
An in vivo animal study and a prospective clinical study have indicated that bone marrow aspirate (BMA) augments spinal arthrodesis
mesenchymal stem cells (MSCs) (CD34−/CD29+/CD105+) isolated from peripheral blood (PB) and BMA The proportion of CD34−/CD29+/CD105+-nucleated cells in the PB was significantly lower than that in the BMA (Figure 1, 0.06% ± 0.03% vs. 0.24% ± 0.06%, p < 0.01, n = 4)
The results suggested that the proportion of MSC-like cells (CD34−/CD29+/CD105+) in the PB was significantly lower than that in the BMA, which provides quantified information for the osteopromotive effects of BMA (Figure 1)
Summary
An in vivo animal study and a prospective clinical study have indicated that bone marrow aspirate (BMA) augments spinal arthrodesis. Autologous bone graft is the gold standard for spinal fusion. Autologous cancellous bone harvested from the ilium is commonly used in intertransverse fusion of the lumbar spine. The incidence of complications associated with harvesting bone from the posterior iliac crest is significant. Bone marrow aspirate (BMA) is harvested percutaneously from a cancellous-rich site such as the iliac crest, proximal tibia, or calcaneus utilizing a bone marrow needle and large-gauge syringe. Animal studies have proven that BMA exhibits osteopromotive properties that promote spinal fusion due to the presence of osteoprogenitor cells and BMPs [8,9]. Our previous prospective clinical study indicated that BMA augments spinal arthrodesis [10]
Sign-in/Register to view highlights & summary Talk to us
Join us for a 30 min session where you can share your feedback and ask us any queries you have
Schedule a callDisclaimer: All third-party content on this website/platform is and will remain the property of their respective owners and is provided on "as is" basis without any warranties, express or implied. Use of third-party content does not indicate any affiliation, sponsorship with or endorsement by them. Any references to third-party content is to identify the corresponding services and shall be considered fair use under The CopyrightLaw.
