Identification of <em>Clostridium perfringens</em> and <em>Fusobacterium necrophorum</em> isolates

Abstract

The article is devoted to the differential diagnosis of strains of Clostridium perfringens and Fusobacterium necrophorum in the studied material of cattle. The research topic is relevant, since rational, timely and accurate diagnosis of the etiology of infectious diseases in cattle in the conditions of industrial cattle breeding prevents the occurrence and transmission of infectious agents, thereby ensuring cost-effective uninterrupted production of highly nutritious products and raw materials. Timely and accurate diagnosis allows veterinarians to prescribe an effective treatment and prevention plan to prevent the occurrence and spread of infectious diseases in cattle, including intestinal diseases [2,6]. One of the causes of hemorrhagic enteritis, reduced productivity and mortality from 25% of young animals may be enterotoxemia associated with toxin-producing strains of Clostridium perfringens. Differential diagnosis is an obligatory step in a comprehensive diagnosis, taking into account epizootological data, clinical signs of sick and conditionally sick animals, pathoanatomical data of forcedly killed and dead animals, laboratory diagnostic methods, including bacteriological and molecular genetic methods. As a result of the study, optimal methods of laboratory diagnostics were applied, including bacterioscopy, the method of pure cultures and polymerase chain reaction (PCR), which made it possible to identify strains of Fusobacterium necrophorum and Clostridium perfringens. Accurate and rapid identification of pathogens Fusobacterium necrophorum and Clostridium perfringens will allow veterinarians to develop a plan of treatment and preventive measures in a short time.