Abstract
Eight ethanol‐tolerant (17% v/v) strains of Leuconostoc were isolated from South African fortified wines which underwent spoilage in the bottle. The levels of genotypic relatedness of these strains to Leuconostoc oenos, Leuconostoc lactis, Leuconostoc argentinum, Leuconostoc mesenteroides subsp. mesenteroides, Leuconostoc mesenteroides subsp. dextranicum and Leuconostoc paramesenteroides (recently reclassified as Weissella paramesenteroides) were determined by performing numerical analysis of total soluble cell protein patterns and DNA‐DNA hybridizations. The fortified wine strains grouped at r≥ 0.90 into one cluster with the reference strains of Leuc. oenos and shared a DNA homology of 82–95% with the type strains of Leuc. oenos (NCFB 1674T). The fortified wine strains were genotypically unrelated to the non‐acidophilic Leuconostoc spp.
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