Abstract
The purpose of this study was to evaluate μ- and δ-opioid receptors (OR) on human and murine dendritic cells (DC). Expression of μ- and δ-OR mRNA on DC was demonstrated by RT-PCR. The immunocytochemical and Western blot analyses revealed the expression of OR protein in DC. Radioreceptor assay demonstrated the specific saturated temperature-dependent binding of [ 3H]-labeled opioid ligand on DC and B max=2.8±0.3 fmol/10 6 cells and K D=4.8±1.0 nM were calculated by a Scatchard analysis. Finally, OR ligands DADLE and DAGO dose-dependently modulated the capacity of DC to induce T cell proliferation in an MLR assay. Importantly, expression of functional OR on DC was significantly increased upon TNF-α-induced DC maturation. Thus, these data suggest a new mechanism of opioid-dependent neuroendocrine immunomodulation.
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