Identification of Anaerosporobacter sp. using next‐generation DNA sequencing as a presumptive cause of chronic cloacitis in a Congo African grey parrot (Psittacus erithacus)

Abstract

AbstractA 29‐year‐old, female Congo African grey parrot (Psittacus erithacus) was presented for a 2‐month history of soft malodorous stool. Next‐generation DNA sequencing of the faecal matter identified Anaerosporobacter sp. in the stool sample. The bird was treated with a 14‐day course of metronidazole, after which next‐generation DNA sequencing no longer detected Anaerosporobacter sp. The bird's clinical signs resolved concurrently. Clinical signs of malodorous stool recurred, and repeated next‐generation DNA sequencing showed recurrence of Anaerosporobacter sp. Duodenal dysmotility/hypermotility syndrome was noted on fluoroscopy. The bird also tested positive for bornavirus and anti‐ganglioside antibodies. We suspect that dysmotility and hypermotility of the duodenum were associated with active bornavirus infection, which caused immunosuppression and predisposed the bird to intestinal colonisation by a novel organism. This report demonstrates the presumptive diagnosis and treatment challenges of Anaerosporobacter sp. from the faeces of a psittaciform species using next‐generation DNA sequencing as opposed to traditional culture techniques.