Abstract
Emergence of New Delhi metallo-β-lactamase-producing Enterobacteriaceae has become a challenging threat to public health. Two carbapenem-resistant Escherichia coli, strain QD28 and QD29, were recovered from the aspirating sputum of a neonate and the urine of an adult in a Chinese hospital in 2013. Molecular typing revealed that both isolates belonged to the sequence type 167, but they were clonally diverse. Both isolates exhibited resistance to carbapenems, cephalosporins, ciprofloxacin, gentamicin, piperacillin-tazobactam and trimethoprim-sulfamethoxazole. In addition, strain QD28 was also resistant to aztreonam, and strain QD29 was resistant to amikacin, fosfomycin and minocycline. Antimicrobial resistance gene screening revealed that strain QD28 harbored aac(6′)-Ib, blaCTX-M-14, blaNDM-5, blaTEM-1 and sul1 genes, and strain QD29 harbored aac(6′)-Ib, blaCTX-M-3, blaNDM-5, blaTEM-1, rmtB, sul1 and sul2 genes. The blaNDM-5 gene was found to be located on a 46-kb plasmid in two isolates, and further sequence analysis showed that this plasmid was highly similar to the previously reported IncX3 plasmid pNDM-MGR194 in India. This is the first identification of blaNDM-5-carrying E. coli in the neonatal infection.
Highlights
It has two amino acid substitutions (Val88Leu and Met154Leu) in comparison with NDM-1, and seems to confer increased resistance to extended-spectrum cephalosporins and carbapenems
Because NDM-5 producers are infrequently detected worldwide, we performed multilocus sequence typing (MLST) and pulsed field gel electrophoresis (PFGE) experiments to analyze their clonal relatedness. Both isolates belonged to the ST167 epidemic clone, but PFGE revealed that they can be classified into different pulsotypes (Fig. 1), indicating that they were clonally diverse and excluding the possibility of nosocomial cross-transmission
Our study reported two NDM-5-producing E. coli isolates from Chinese hospitalized patients in 2013
Summary
It has two amino acid substitutions (Val88Leu and Met154Leu) in comparison with NDM-1, and seems to confer increased resistance to extended-spectrum cephalosporins and carbapenems. Different sequence types (STs) of E. coli and K. pneumoniae have been detected as NDM-5 producers. The diversity of these isolates indicates probable horizontal transfer of the blaNDM-5 gene either through plasmids or the transposon-related mobile elements. Considering its increased resistance phenotypes and global distribution, epidemiological survey of blaNDM-5 should arouse our attention. We detected two NDM-5-producing E. coli isolates from the hospitalized patients in a Chinese hospital, of which one strain was isolated from a neonate and resistant to a range of antimicrobials
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