Abstract
<p> <span style="font-size: medium;">Alkaloids are a group of natural compounds originally found in plants. <em>Suffruticosa</em> is a </span><span style="font-size: medium;">species</span><span style="font-size: medium;"> of the genus <em>Semenovia</em>, the <em>Apiaceous</em> family, a native herb in Iran. In this family, alkaloids are found rarely. Humic substances application to the root soil at the beginning of plant growth improves quality, productivity and physical properties of the soil. Accordingly</span>, <span style="font-size: medium;">humic substances were added separately to the root soil of the samples and other samples were selected as the blank plant sample in the distance of 50 cm. Methanol extracts were prepared from dried and powdered leaf samples. The crude alkaloid solution was extracted from methanol extract with 2 % sulfuric acid, dense ammonia and chloroform. The crude alkaloid solution was passed gradiently with mixture solvents of methanol and chloroform through a chromatography column. The output samples of the chromatography column were analyzed by FT-IR, UV-Vis, and TLC and GC-MS techniques. Pyrimido [1, 2-a] quinoxalinone and 1, 3,8-triisopropyl-6-methyl-2,7-naphthyridine alkaloids were identified in both samples and the extracted humic acid (0.513% and 0.979% </span>respectively)<span style="font-size: medium;"> and the Sapropel solution (0.449% and 0.678% </span>respectively<span style="font-size: medium;"> )were added to their plants soil. Also another alkaloid named 9-benzyloxypyrido [1,2-a]pyrimidin-4-one(9.538%) was identified in the sample and Sapropel was sprayed onto its root soil. No alkaloid was found in the blank plant sample. The results showed that humic substances had considerable effects on the effective substances of the plant <em>Semenovia.suffruticosa</em>.</span></p><p><em><span style="font-size: medium;"> </span></em></p>
Highlights
The genus Semenovia has 11 species in Iran [1]
Identifying the percentage of the classification compounds indicates that their variations have been affected by addition of Humic substances (HS) compared to all the four samples (the blank sample and the three samples, before and after their column chromatography (CC) in which HS was added to their root soil (Tables 1, 2 and Figure 1)
The percentage of oxygenated sesquiterpenes in the alkaloid solution of plant with Sapropel in root after Chromatography column (CC) and the alkaloid solution of plant with Sapropel at root before CC and the alkaloid solution plant with humic acid (HA) in root after CC were decreased to 0.713%, 0.563% and 1.174%, respectively, compared to the alkaloid solution of the blank sample with a percentage of 0.669.Oxygenated terpenoids, coumarin and all of terpenoids were increased significantly when HA was added to the soil compared with other samples.The total percentages of the identical alkaloids were 1.491% and 10.919% in the plants to the soil of which HA and Sapropel solution were added
Summary
The genus Semenovia has 11 species in Iran [1]. Semenovia suffruticosa is a perennial plant species of Apiaceae (Umbeliferae) family that grows only in altitudes of 2300 - 2500m Taftan Mountain (Sistan & Baluchestan province, Iran). The plant Semenovia suffruticosa has comb-like leaves or bifurcate divisions and long petioles with hard pods. The stems are almost cylindrical trunk and 45 70 cm long, with low bifurcate branches, and sometimes with shallow grooves on the surface. Terminal Umbels are within 7 10 cm radiuses with almost equal parts and glabrous, and 40 - 50 cm long in fruit-containing state Umbellules have 12 - 15 flowers and peduncle is shorter than the ripe fruit [2]. Apiaceae family plants contain compounds such as coumarin, furanocoumarin, cromenocoumarin, terpene, sesquiterpene, triterpenoid saponins and acetylene compounds.
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