Abstract
PurposeTo identify the aberrantly expressed long non‐coding RNAs (lncRNAs) in ovarian high‐grade serous carcinoma (HGSC).MethodsTotal RNA was isolated in HGSC cell lines, ovarian surface epithelial cells, and normal ovaries. Aberrantly expressed lncRNAs in HGSC were identified by PCR array, which analyzes 84 kinds of lncRNAs. To infer their functions, HGSC cell lines with different levels of expression of the identified lncRNAs were established, and then, activities of proliferation, migration, and apoptosis were examined. Expression levels of the identified lncRNAs were also examined in multiple ovarian HGSC tissues.ResultsTen aberrantly expressed lncRNAs, six upregulated and four downregulated, were identified in the HGSC cell lines. The authors established four HGSC cell lines: in two of the cell lines, one of the upregulated lncRNAs was knocked down, and in two other cell lines, one of the downregulated lncRNAs (MEG3 and POU5F1P5) was overexpressed. Migration activities were inhibited in the HGSC cell lines overexpressing MEG3 or POU5F1P5 while there were no differences in proliferation and apoptosis between the established and control cell lines. The four lncRNAs downregulated in the HGSC cell lines were also observed to be downregulated in ovarian HGSC tissues.ConclusionThe authors identified four downregulated lncRNAs in ovarian HGSC.
Highlights
Ovarian cancer is the most lethal gynecologic cancer
We focused on high-grade serous carcinoma (HGSC), which are the most frequently occurring and aggressive subtypes among ovarian cancers, and aimed to identify long non-coding RNAs (lncRNAs) involved in the progression and malignant behaviors of HGSC
The HGSC cell lines that stably overexpressed each of the downregulated lncRNAs (MEG3 and POU5F1P5) were established by transducing their expression vectors into the cells (Figure 2B)
Summary
Ovarian cancer is the most lethal gynecologic cancer. Based on histopathology and molecular genetics, epithelial ovarian cancers (EOCs) are mainly classified into endometrioid, clear cell, mucinous, lowgrade serous, and high-grade serous carcinomas (HGSC).[1,2,3] HGSC is the most aggressive subtype and is responsible for approximately two-thirds of total EOCs.[2]. The number of publications related to the biological roles of lncRNAs in cancers has increased exponentially in the past few years.[7,10] In ovarian cancer, the lncRNAs such as H19, HOTAIR, HOXA11-AS, LSINCT5, MALAT1, and PVT1 have been reported to be involved in cell proliferation and malignancy.[10,11,12,13]. We focused on HGSCs, which are the most frequently occurring and aggressive subtypes among ovarian cancers, and aimed to identify lncRNAs involved in the progression and malignant behaviors of HGSC. Ten cases of normal ovarian tissues were obtained from the patients who underwent oophorectomy by a reason different from HGSC at Yamaguchi University hospital. The relative expression levels were calculated with the delta-delta Ct method using GAPDH as a reference gene
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