Abstract
Porcine reproductive and respiratory syndrome virus (PRRSV) is one of the most economically important pathogens, that hinder the development of global pork industry. Its nonstructural protein 11 (nsp11), with the nidoviral uridylate-specific endoribonuclease (NendoU) domain, is essential for PRRSV genome replication and it also contributes to host innate immunity suppression. However, the immunogenicity and immune structure of PRRSV nsp11 have not been well investigated yet. In this study, a monoclonal antibody (mAb), designated 3F9, that against nsp11 was generated. Subsequently, a series of partially overlapped fragments, covered the nsp1140-223aa, were expressed to test the reactivity with mAb 3F9, and the 111DCREY115 was found to be the core unit of the B-cell epitope recognized by mAb 3F9. Further investigation indicated that both genotype 1 and genotype 2 PRRSV can be recognized by mAb 3F9, due to the 111DCREY115 is conserved in both genotype virus. Meanwhile, this epitope, localized at the surface of nsp11 in 3D structure, is confirmed to be able to induce humoral immune response in PRRSV infected pigs. These findings do not only provide an mAb tool to further investigate the function of nsp11, they also indicate the diagnostic potential for this epitope.
Highlights
Porcine reproductive and respiratory syndrome (PRRS) is characterized as widespread reproductive failures in pregnant sow, including mummified, stillborn and aborted fetuses, as well as respiratory distress in all age pigs
Following isotyping results showed that monoclonal antibody (mAb) 3F9 belongs to subclass IgG1/κ-type
To further confirm the specificity of the mAb 3F9, the porcine reproductive and respiratory syndrome virus (PRRSV) JXwn06 infected MARC-145 cells were analyzed by western blotting (WB) and IFA using the mAb 3F9 as the primary antibodies
Summary
Porcine reproductive and respiratory syndrome (PRRS) is characterized as widespread reproductive failures in pregnant sow, including mummified, stillborn and aborted fetuses, as well as respiratory distress in all age pigs. Since it first emerged in the United States in late 1980s, as a “mystery” disease, PRRS had spread to most pig raising area of the world, which badly hinders the development of global pork industry [1,2,3,4]. Identify a conserved B-cell epitope in PRRSV nsp. The funders had no role in study design, data collection and analysis, decision to publish, or preparation of the manuscript
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