Abstract
Glycerophosphodiester phosphodiesterase (GDPD/GDE) catalyzes the hydrolysis of glycerophosphodiesters to glycerol 3-phosphate and alcohol. It was discovered that the glycerophosphodiesterase family plays a role in lipid metabolism and signal pathway in recent years, but little has been known about the characteristics of chicken GDEs. Here, chicken GDE5 (cGDE5) was identified and characterized for the first time. The full length coding cDNA sequence of cGDE5 was cloned, which encoded a polypeptide with 678 amino acids containing a carbohydrate-binding module 20 (CBM20) and a GDPD domain. Tissue expression profiles showed that cGDE5 mRNA was high in various tissues. such as heart, brain, skeletal muscle and testis. Moreover, cGDE5 was demonstrated to exhibit glycerophosphocholine phosphodiesterase activity. These results together suggested that cGDE5, as a unique member of GDE family, may play multiple roles as a cytoplasmic glycerophosphocholine phosphodiesterase.
Highlights
Two important factors considered by the poultry industry are meat yield and quality
These results indicated GDE5 played a key role in muscle and adipose tissue development
Protein domain analysis showed that chicken GDE5 (cGDE5) had a carbohydrate-binding module 20 (CBM20) in the N-terminal region and a GDPD domain in the C-terminal region (Fig. 1B)
Summary
Two important factors considered by the poultry industry are meat yield and quality. At present, chickens are marketed in about half the time and at about twice the body weight compared to 50 years ago, which is closely related to a significant increase in the muscle proportion[1]. Mouse GDE5 was initially identified to be a GPC phosphodiesterase and controls skeletal muscle development[8]. GDE5DeltaC471 that contains GDE sequence but lacks GPC phosphodiesterase activity innegatively regulates skeletal muscle development[9]. Adipose tissue weight and blood triglyceride levels were increased in the transgenic mice expressing GDE5DeltaC471 in skeletal muscle[10]. These results indicated GDE5 played a key role in muscle and adipose tissue development. The chicken is an important animal for providing meat, but it is serves as a valuable avian model. Chicken cGDE5 was first cloned and built a unique 3-D molecular model. CGDE5 tagged with green fluorescence protein (GFP) was observed to be a cytological protein and identified to have GPC-hydrolyzing activity
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