Abstract
The gene lac-1, encoding the enzyme laccase, is the best characterized of a number of genes in the chestnut blight fungus, Cryphonectria parasitica, that are repressed by hypoviruses, a group of virulence-attenuating mycoviruses. lac-1 has also been shown to be transcriptionally activated by low concentrations of the translational inhibitor cycloheximide (CHX) and by the immunosuppressant cyclosporin A. We now report the identification of a CHX responsive element within the lac-1 promoter region. Gel-mobility shift analysis revealed a 111-bp fragment located 1.8 kb upstream of the lac-1 transcriptional start point that exhibited protein binding activity. Insertion of this element within a basal lac-1 promoter sequence conferred CHX responsive transcriptional activation. Moreover, this activation was prevented by hypovirus infection. A 22-bp sequence with an imperfect dyad symmetry located within the 111-bp element was found to be essential for sequence-specific protein binding and, thus, represents a putative target for interactions between the lac-1 promoter and proteins that are involved in mediating CHX inducible activation of lac-1 transcription.
Sign-in/Register to access full text options 
Talk to us
Join us for a 30 min session where you can share your feedback and ask us any queries you have
Schedule a callDisclaimer: All third-party content on this website/platform is and will remain the property of their respective owners and is provided on "as is" basis without any warranties, express or implied. Use of third-party content does not indicate any affiliation, sponsorship with or endorsement by them. Any references to third-party content is to identify the corresponding services and shall be considered fair use under The CopyrightLaw.
