Abstract
Cytochrome P-450, subfamily I, polypeptide 1 (CYP1A1) encodes a member of cytochrome P450 superfamily of enzymes. This protein localizes to the endoplasmic reticulum and its expression is induced by some polycyclic aromatic hydrocarbons (PAHs), some of which are found in cigarette smoke. To understand the core sequence within the 5′ regulatory region of porcine CYP1A1 gene, nine primer pairs were designed to amplify the different length of the CYP1A1 5′ regulatory region, then the PCR products were constructed to the Firefly luciferase gene report vector PGL3-basic. Detection results in PK15 cell and fibroblasts all showed that a 39 bp fragment (−68 bp – −29 bp upstream) was the core sequence of porcine CYP1A1 promoter. Further research found that HSF and SRY might be the most important transcriptional factor for porcine CYP1A1 promoter.
Sign-in/Register to access full text options 
Talk to us
Join us for a 30 min session where you can share your feedback and ask us any queries you have
Schedule a callMore From: Journal of Northeast Agricultural University (English edition)
Disclaimer: All third-party content on this website/platform is and will remain the property of their respective owners and is provided on "as is" basis without any warranties, express or implied. Use of third-party content does not indicate any affiliation, sponsorship with or endorsement by them. Any references to third-party content is to identify the corresponding services and shall be considered fair use under The CopyrightLaw.
