Identification, Determination and Spectral Study

Abstract

Corona viruses resist many antiviral agents which reduces the antiviral therapies. Remdesivir is an antiviral drug efficient against single-stranded RNA viruses. Analytical determinations of this drug are very benefit in selecting the correct dose and getting real information and formulas of this compound. In this work, remdesivir has been identified at 239 nm in aqueous alkaline (1M NaOH), at 245 nm in ethanol, and at 246 nm. in methanol, it has also been followed at 239 nm using the alkaline medium and determined in injection, the linearity range was from 1to 60 µg/ml with molar absorptivity 2.036×104 l.mol-1.cm-1, and Sandell’s sensitivity index is 0.0295 µg.cm-2, LOD is 0.2613 and LOQ is 0.871 µg/mL. I. R spectrum of solid remdesivir shows band at 1639.96 cm -1 due to the carbonyl group and at 3350.79 cm-1refer to the hydroxy group adjacent to NH2 group which appears at about 3300 cm-1, the aromatic was identified at 1660-2000 cm-1 and C-O of ester at 1153cm-1. These bands in the alkaline medium were slightly shifted to be 1637 cm -1 due to carbonyl group and at 3318 cm-1refer to hydroxy group adjacent to NH2 group which appeared at about 3264 cm-1, the aromatic was identified at 2131 cm-1, and C-O of ester at 1379cm-1.