Many organisms go through a process of programmed cell death called apoptosis while newer cells are created. This has the effect of protecting the organism from cellular parasites and is a major line of defense against invading organisms. Apoptosis inhibitors, then, play an important role in aiding infectious agents by inhibiting caspase protease and thus the apoptopic pathway. In this study, we identified an inhibitor of apoptosis protein (IAP) in the microsporidian Nosema bombycis (NbIAP). NbIAP a composed of 218 amino acids containing two overlapping domains; the BIR domain and a zf-C3HC domain. We show, through indirect immunofluorescence, that NbIAP is present throughout the life cycle of N. bombycis and is localized in the nucleus of the parasite and therefor does not act on caspase protease directly. qRT-PCR analysis shows that the expression of the NbIAP gene was the highest on the first day of infection, then decreased to a relatively stable level. In addition, we show that the downregulation of the NbIAP gene directly inhibits the proliferation of N. bombycis. These findings suggest that NbIAP plays an important role in the N. bombycis life cycle.
Microsporidian Nosema Bombycis First Day Of Infection Inhibitor Of Apoptosis Protein BIR Domain Caspase Protease Stable Level Apoptosis Inhibitors Indirect Immunofluorescence Life Cycle Inhibitor Of Protein
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Round-ups are the summaries of handpicked papers around trending topics published every week. These would enable you to scan through a collection of papers and decide if the paper is relevant to you before actually investing time into reading it.
Climate change Research Articles published between Jan 23, 2023 to Jan 29, 2023
Jan 30, 2023
Articles Included: 3
Climate change adaptation has shifted from a single-dimension to an integrative approach that aligns with vulnerability and resilience concepts. Adapt...Read More
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