Abstract
The identification of N-alkylamides from commercial Acmella oleracea extract, their UV-B photostability in different solvents, and identification of degradation products were the main goals of this study. By UHPLC-DAD-ESI-MS/MS method the presence of nine N-alkylamides was identified. Investigation of UV-B irradiation effect on identified N-alkylamides from Acmella oleracea extract was monitored in various the most commonly used solvents (methanol, ethanol, saline solution, and water) during 120 min. The results obtained indicated that spilanthol and homospilanthol were the most stable N-alkylamides presented in Acmella oleracea extract, while the photostability of identified N-alkylamides in whole in tested extract solutions decreased as follows: methanol>ethanol>saline solution>water. As the main degradation products in all investigated solutions 6,9-dihydroxy-deca-2,7-dienoic acid isobutyl-amide and 8,9-dihydroxy-deca-2,6-dienoic acid isobutyl-amide were identified.
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