Abstract
Accurate detection and recognition of chemical signals play extremely important roles for insects in their survival and reproduction. Chemosensory receptors, including odorant receptors (ORs), ionotropic receptors (IRs) and gustatory receptors (GRs), are involved in detection of volatile signals. In the present study, we aimed to identify candidate chemosensory receptors, and RNA-seq technology was employed to sequence the antennal transcriptome of Protaetia brevitarsis (Coleoptera: Scarabaeidae: Cetoniinae), a native agricultural and horticultural pest in East-Asia. According to the sequence similarity analysis, we identified 72 PbreORs, 11 PbreGRs and eight PbreIRs. Among PbreORs, PbreOR2, PbreOR33 and PbreOR53 were preliminarily classified into pheromone receptors. Further qRT-PCR analysis indicated that 11 PbreORs were specifically expressed in the antennae of male P. brevitarsis, whereas 23 PbreORs were specifically expressed in the female antennae. Our results laid a solid foundation for further functional elucidations of insect chemoreceptors, which could be used as the potential targets of pest management.
Highlights
A sophisticated olfactory system is crucially important for survival and reproduction of most insects[1]
The larvae of P. brevitarsis have not been documented as an underground pest, because their grubs are saprophytic, inhabiting in decaying plant tissue or fermented animal manure
According to the neighbor-joining tree of ionotropic receptors (IRs) from six coleopterans, we found that PbreIR25a, PbreIR93a, PbreIR8a, PbreIR21a, PbreIR40a, PbreIR76b and PbreIR75a were classified into IR25a, IR93a, IR8a, IR21a, IR40a, IR76b and IR75a lineages with high bootstrap values, respectively (Fig. 6)
Summary
A sophisticated olfactory system is crucially important for survival and reproduction of most insects[1]. The main olfactory organs in odorant recognition, there are several types of olfactory proteins, which are directly involved in the procedures of detection, recognition or degradation of peripheral odorants These olfactory proteins include odorant-binding proteins (OBPs)[2,3], chemosensory proteins (CSPs)[4,5], odorant receptors (ORs), ionotropic receptors (IRs)[6], gustatory receptors (GRs)[7], sensory neuron membrane proteins (SNMPs)[8] and odorant-degrading enzymes (ODEs)[9]. Www.nature.com/scientificreports to olfactory-related genes of many insects in Coleoptera have been systematically studied, including species in Tenebrionidae, Curculionidae, Cerambycidae, Bothrideridae, Bostrychidae, Chrysomelidae and Scarabaeidae[17,18,19,20,21,22,23,24,25,26,27,28,29,30,31]. Our findings could help better understand the mechanisms of olfactory recognition in P. brevitarsis, and provide a solid foundation for further study on relationship between olfactory-related genes and semiochemicals
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