Abstract
Naturally, the fertility of Aceh cattle has declined and caused huge losses in the development of Aceh cow germplasm. Several genetic-based studies have been carried out in Aceh cattle, including characteristics of genetic diversity using microsatellite DNA and its relationship to body weight in Aceh cows, but there is no research on genomics that is closely related to fertility, especially fertility in aceh cows. Previous studies have revealed a correlation between fertility with the expression of several genes involved in spermatogeneses such as Protamines (PRM 1, 2, and3) and Transition Nuclear Proteins (TNPS 1 and 2). This study aims to identify thePRM1gene that plays an important role in spermatogenesis and the fertility status of Aceh cattle. This research was carried out using 10 Aceh cattle testes taken from slaughterhouses, followed by DNA isolation, amplification of thePRM1gene, DNA sequencing, and DNA base sequence analysis. The result showed that the nucleotide sequences of aceh cattlePRM1gene did not differ from thePRM1gene fromBos taurusandBos indicusin the GeneBank. Analysis of protein sequences showed thatPRM1in Aceh cattle sperm is rich in the amino acid arginine, which is 36.92%. From this study, it can be concluded that thePRM1 genefrom Aceh cattle is identical to thePRM1gene fromBos taurusandBos indicusboth at the DNA sequence level and at the protein sequence.
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