Abstract

Eleven different species of yeasts isolated from raw milk, such as Issatchenkia orientalis, Pichia fermentans, Rhodotorula mucilaginosa and Yarrowia lipolytica were identified using an integrated approach, including phenotypic and genotypic methods and the API 20C AUX system. Eleven selected strains which tolerated low pH levels, gastric juice and bile salt were further analysed for the presence of functional traits useful for probiotic application as antioxidants. The ability to scavenge DPPH (2, 2‐diphenyl‐1‐picrylhydrazyl) and inhibit linoleic acid peroxidation indicated that P. fermentans BY5 and HJ15 may be promising candidate strains for use as probiotics with antioxidant activity.

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