Abstract
Nucleus pulposus-derived mesenchymal stem cells (NPMSCs) have shown a good prospect in the regeneration of intervertebral disc (IVD) tissues. However, fresh NPMSCs are not always readily available for basic research and clinical applications. Therefore, there is a need for an effective long-term cryopreservation method for NPMSCs. The aim of this study was to determine whether adding icariin (ICA) to the conventional cryoprotectant containing dimethyl sulfoxide (DMSO) had a better cryoprotective effect for NPMSCs. The results showed that the freezing solution containing ICA along with DMSO significantly increased the postthawed cell viability, decreased the apoptosis rate, improved cell adherence, and maintained the mitochondrial functions, as compared to the freezing solution containing DMSO alone. And the inhibition of oxidative stress and upregulation of heat shock proteins (HSPs) in the presence of ICA also confirmed the beneficial effect of ICA. Furthermore, ICA had no cytotoxicity and did not alter the characteristics of postthawed NPMSCs. In conclusion, these results suggested that the addition of ICA to the conventional freezing medium could improve the viability and function of the cryopreserved human NPMSCs and provided an optimal formulated freezing solution for human NPMSC cryopreservation.
Highlights
Low back pain (LBP) is one of the most common health problems throughout the world, which creates heavy financial burden globally [1]
The results demonstrated that the viability of nucleus pulposus-derived mesenchymal stem cells (NPMSCs)
For NPMSCs cryopreserved with dimethyl sulfoxide (DMSO) and ICA, ICA (12.5–100 μM) significantly increased the viability of postthawed NPMSCs (P < 0 05), with maximum viability observed at a concentration of 25 μM. The concentration (25 μM)
Summary
Low back pain (LBP) is one of the most common health problems throughout the world, which creates heavy financial burden globally [1]. Intervertebral disc (IVD) degeneration is considered as the main cause of LBP [2]. Surgical operations and conservative treatments for IVD degeneration are not long-lasting and effective for the limitation that they cannot restore the IVD tissues [3]. Supplementation with exogenous mesenchymal stem cells (MSCs), such as MSCs derived from bone marrow and adipose tissue, has shown positive outcomes for the repair of IVD degeneration [4]. Evidence has been found that endogenous nucleus pulposus-derived mesenchymal stem cells (NPMSCs) exist naturally in the IVDs [5,6,7]. As a novel approach to repairing disc degeneration, application of endogenous NPMSCs has shown exciting prospect and attracted increasing attention [8, 9]. The effective and long-term preservation of NPMSCs is crucial for the application of NPMSCs
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