HYPOGLYCEMIC EFFECT OF OLIVE MILL WASTEWATER IN MICE INVOLVES THE SGLT-1 TRANSPORTER

Abstract

<p><strong>Objective: </strong>Olive mill wastewater (OMWW) is a by-product of olive oil production and contains biologically active compounds. The aim of this study was to investigate the effect of OMWW on intestinal glucose absorption in mice, a target for treating diabetes.</p><p><strong>Methods: </strong>The effect oral administration of OMWW on mice glycemia was evaluated<em> </em>by oral glucose tolerance tests (OGTT), and mice jejunal mucosal strips were mounted in Ussing chambers to evaluate the activity of sodium-dependent glucose transporter (SGLT-1). Western blot analysis was performed to study SGLT-1 and protein kinase C (PKC) at the brush border membranes (BBM) of mice jejunal mucosa.</p><p><strong>Results: </strong>We found that the raised glycemia in mice in response to oral glucose administration (OGTT) was decreased in presence of OMWW, being highly effective at 4 g/kg of body weight. Further, OMWW inhibited glucose induced short-circuit current (Isc) in mice jejunal, which is a marker of SGLT-1 activity. The effect was rapid, with maximal inhibition of 80 % versus control. Also, OMWW induced a strong decrease of SGLT-1 protein (60%) as well as PKC (30%), a key effector protein in BBM of mice jejunal mucosa in response to glucose.</p><p><strong>Conclusion: </strong>Our results suggest that OMWW<em> </em>may be beneficial for diabetes through its novel hypoglycemic effect by acting on the PKC-SGLT-1 axis.</p>