Hydrogen-containing preservation solution relieves oxidative stress and inflammatory damage of rat donor heart

Abstract

Objective To study the effect of hydrogen-containing preservation solution against oxidative stress and inflammatory damage of rat donor heart. Methods Thirty -two SD rats were evenly randomized into four groups( n = 8) :control group ( the hearts were protected by HTK solution) ,H1 group ( hydrogen concentration was about 0. 2 mmol/L in the HTK solution) ,H2 group ( hydrogen concentration was about 0. 4 mmol/L in the HTK solution) and H3 group ( hydrogen concentration was about 0. 8 mmol/L in the HTK solution). The rat hearts were harvested in all groups and were mounted on the Langendorff apparatus to estimate baseline hemodynamic values. All the hearts underwent hypothermic ( 4℃) storage for 6 h in the corresponding cardioprotective solutions. Then,superoxide dismutase ( SOD) activities and malondiadehyd ( MDA) contents in myocardium tissues were measured after reperfusion by xanthine oxidase method and TBA method,respectively. The levels of 8-hydroxydeoxygunosine,TNF -α,and IL-6 in the myocardium tissues were measured by enzyme-linked immunosorbent assay ( ELISA). Results The MDA levels in H1,H2,and H3 groups were lower than that in the control group 6 h after preservation ( P 0. 01 or P 0. 05) ,and the SOD activities in H2,H3 groups were higher than that in the control group ( P 0. 01 or P 0. 05). The levels of 8 -hydroxydeoxygunosine,TNF -α,and IL -6 in the control group were higher than those in H1,H2,and H3 groups ( P 0. 01 or P 0. 05). Conclusion Hydrogen-containing preservation solution can relieve the oxidative damage and reduce production of inflammation factors during preservation of rat donor heart.