Abstract
The HA is present in almost all vertebrates and plays a critical role in tissue development and cell proliferation, it has been demonstrated to promote wound healing and involved in angiogenesis and inflammation. Also polynucleotydes (PN) have proved to promote the “in vitro” growth and activity of human fibroblasts and osteoblasts, to increase reparation on UVB damaged dermal fibroblasts and seems to promote proliferation of human pre-adipocytes. Several in vivo studies have demonstrated the PN effect also in vivo, inducing an increase of angiogenesis and healing process. In this paper we have evaluated the effect of a mixture of Polynucleotides (PN) and entire Hyaluronic Acid (HA) on cultured human fibroblasts by analyzing cell growth. Different mixture have been tested and it has been demonstrated that the presence of HA even at low concentration (1 mg/ml) determine an increase of PN activity up to 20%. Furthermore, the addition of HA 1 mg/ml to PN 100 μg/ml induces a cell growth rate comparable to that exerted by PN concentration of 12 μg/ml.
Highlights
Large body of evidence supports the concept of critical role of microenvironment in cell behavior, the area around cells plays an important action in normal tissues, regulating growth factor concentration, nutrients supply and maintaining an intense cross-talk between cells
In this paper we have evaluated the effect of a mixture of Polynucleotides (PN) and entire Hyaluronic Acid (HA) on cultured human fibroblasts by analyzing cell growth
Some other authors have studied the effect of PN both in in vitro and in vivo: PN have been demonstrated to promote the growth of human corneal fibroblasts [3] and osteoblasts [4], to increase reparation on UVB damaged dermal fibroblasts [5] and seems to promote proliferation of human pre-adipocytes
Summary
Large body of evidence supports the concept of critical role of microenvironment in cell behavior, the area around cells plays an important action in normal tissues, regulating growth factor concentration, nutrients supply and maintaining an intense cross-talk between cells. The drug is obtained by an extraction process with purifying and high temperature sterilizing procedures to obtain a 95% pure active principle without pharmacologically active proteins and peptides This compound holds a mixture of deoxyribonucleotides polymers with chain lengths ranging between 50 and 2000 bp and may represent the source of purine and pyrimidine deoxynucleosides/deoxyribonucleotides and bases. HA determine a reduction of cartilage degradation, it has been demonstrated that that expression of matrix metalloproteinase-3 (MMP-3) and IL-1beta were suppressed in synovium but not in cartilage tissues of the rabbit ACLT model by treatment with HA84, even though the suppressive effect on the articular cartilage destruction was still observed.
Sign-in/Register to view highlights & summary Talk to us
Join us for a 30 min session where you can share your feedback and ask us any queries you have
Schedule a callMore From: Journal of Cosmetics, Dermatological Sciences and Applications
Disclaimer: All third-party content on this website/platform is and will remain the property of their respective owners and is provided on "as is" basis without any warranties, express or implied. Use of third-party content does not indicate any affiliation, sponsorship with or endorsement by them. Any references to third-party content is to identify the corresponding services and shall be considered fair use under The CopyrightLaw.
