Abstract
High-affinity metal binding by isolated humic acids has been observed for both copper and calcium in metal titration experiments. Results of titrations of humic acids with a single metal (either calcium or copper) are consistent with a discrete ligand site model of humate-metal binding. However, copper titrations in the presence of excess calcium do not show competitive effects predicted by such a model. Hence, different ligand sites must be involved in calcium and copper binding, or a binding mechanism other than discrete ligand binding must be operative.
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