Abstract
Subzonal sperm insemination (SUZI) into hamster oocytes was performed to establish the karyotypes of the fertilizing spermatozoa. Spermatozoa from two males with normal semen parameters were microinjected. Of 72 (52 + 20) analyzed sperm chromosome metaphases, only 1 (1.4%) was considered abnormal, showing a structural abnormality. No hyperhaploidy was observed. Rates of sperm chromosomal abnormalities after microinjection were not higher than those reported previously using zona-free egg insemination, suggesting that the SUZI procedure per se does not increase sperm chromosomal abnormalities. The use of subzonal insemination into hamster oocytes for the study of human sperm chromosomes in males with low sperm counts is discussed.
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