Abstract
Localisation of antibody and/or antigen to target cells in human skin was studied by autoradiography. Full-thickness biopsies were obtained 24, 48, and 72 hours following passive local sensitization with 125I-labeled E-myeloma protein. Other biopsies were obtained 5, 15, and 30 minutes following challenge with goat anti-E-myeloma. Sites passively sensitized with ragweed-allergic sera were challenged with 125I-labeled ragweed antigen E, and biopsies were obtained at 5, 15, and 30 minute intervals. Forty-eight and 72 hours following passive sensitization the E-myeloma protein was found to be exclusively associated with skin mast cells. In some biopsies, obtained 24 hours following passive sensitization in addition to mast cells, E-myeloma was localized to other cell types as well. Biopsies of the wheal-and-flare reaction sites revealed that the antigen-induced release of histamine from the human skin mast cells results in the degranulation of the reacting cells.
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