Human RNA-specific adenosine deaminase ( ADAR1) gene specifies transcripts that initiate from a constitutively active alternative promoter

Abstract

The human ADAR1 gene specifies two size forms of RNA-specific adenosine deaminase, an interferon (IFN) inducible ∼150 kDa protein and a constitutively expressed N-terminally truncated ∼110 kDa protein, encoded by transcripts with alternative exon 1 structures that initiate from different promoters. We have now identified a new class of ADAR1 transcripts, with alternative 5′-structures and a deduced coding capacity for the ∼110 kDa protein. Nuclease protection and 5′-rapid amplification of cDNA ends (5′-RACE) revealed five major ADAR1 transcriptional start sites that mapped within the previously identified and unusually large (∼1.6 kb) exon 2. These transcripts were observed with RNA from human amnion U cells and placenta tissue. Their abundance was not affected by IFN-α treatment of U cells in culture. Transfection analysis identified a functional promoter within human genomic DNA that mapped to the proximal exon 2 region of the ADAR1 gene. Promoter activity was not affected by IFN. These results suggest that transcripts encoding the constitutively expressed ∼110 kDa form of the ADAR1 editing enzyme are initiated from multiple promoters, including one within exon 2, that collectively contribute to the high basal level of deaminase activity observed in nuclei of mammalian cells.