Human Protein Structural Interaction Network: Domain Effects on Network Topology and Protein Function*

Abstract

Availability of high-throughput protein-protein interaction and domain-domain interaction information make it possible to study human structural interaction network, and uncover the inner relationship between structure and function of proteins in proteomics area.The widely-distributed domains are thought to affect structure and function of proteins.However, it is still a challenge to investigate potential mechanisms of these effects combing the structural information (e.g domain number, domain length and domain coverage).The proteins were classified into single- or multi- domain proteins, then human protein structural interaction network was constructed with classification information by integrating the protein-protein interaction and the domain- domain interaction data.Furthermore, comparing with the protein-protein interaction network, specific structure characteristics of human protein structural interaction network were studied. And with respect to single-/multi- domain proteins, function enrichment analysis was carried out for their functions. With domain-domain interaction considered, human protein structural interaction network could provide more detailed information distinct from networks based on protein-protein interaction datasets only, and might reveal the underlying complexity of protein-protein interaction network from the perspective of protein classification. Human protein structural interaction network could exploit domain information to provide additional and crucial protein interaction details necessary for understanding what human structural interactions imply.