Abstract
BackgroundTo investigate the role of prolyl hydroxylase (PH), a key enzyme of collagen synthesis, in human uterine leiomyoma, PH expression was determined in the normal uterine myometrium and the leiomyoma tissues during the menstrual cycle.MethodsThe tissues were obtained from 40 regularly cycling women (aged 29 to 53 yr) who were undergoing abdominal hysterectomy for symptomatic uterine leiomyoma. Immunohistochemistry for human PH with specific monoclonal antibody was used for analysis.ResultsImmunohistochemical staining for PH revealed intense staining of leiomyoma cells in the uterine leiomyoma throughout the menstrual cycle, as compared with the adjacent normal myometrium. In the secretory phase, weak or no immunostaining for PH was detected in the normal myometrial tissues.ConclusionsThese results suggest that increased expression of PH might play an role in the physiology of uterine leiomyoma during the menstrual cycle.
Highlights
To investigate the role of prolyl hydroxylase (PH), a key enzyme of collagen synthesis, in human uterine leiomyoma, PH expression was determined in the normal uterine myometrium and the leiomyoma tissues during the menstrual cycle
The precise control of extracellular matrix (ECM) metabolism in the uterine myoma and myometrium is critical for understanding the pathophysiology and development of uterine leiomyoma
The strong expression of hPH was detected, as compared with the normal adjacent myometrium during the menstrual cycle. These findings suggest that leiomyoma cells might synthesize collagens more actively than the normal myometrial cells throughout the menstrual cycle consistently
Summary
To investigate the role of prolyl hydroxylase (PH), a key enzyme of collagen synthesis, in human uterine leiomyoma, PH expression was determined in the normal uterine myometrium and the leiomyoma tissues during the menstrual cycle. The extracellular matrix (ECM) is considered to play an important role in the stability of tissues and in regulating the growth and differentiation of cells [1,2]. It is suggested that ECM plays an ‘informational’ role through a network of interactions between cells and signal molecules that is of primary importance in the control of cellular proliferation and motility during histogenesis, for the maintenance of tissue homeostasis and in cancer development. The ECM of uterine leiomyoma has been studied [4,5,6,7], but changes of the collagen metabolism in uterine tissues are not fully understood. The precise control of ECM metabolism in the uterine myoma and myometrium is critical for understanding the pathophysiology and development of uterine leiomyoma
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