Abstract
Neural cells cultures from human embryo brain of 9° - 11°W gestational age have been used to study ERα (Estrogens Receptor α) and to perform toxicity test for Mitomycin C and Methotrexate. Histochemical confirmation of cellular neuronal phenotype was based on histochemical evidence of NSE (Neuron Specific Enolase).The detection of ERα in neuronal cells was performed with a rabbit Monoclonal Antibody. ERα was absent both on neurons grown in vitro and on tissue brain specimens. This finding is apparently in contrast with the positive immunoreactivity of ERα and ERβ reported by other Authors on foetal and adult CNS (Central Nervous System). The absence of nuclear ERα on neurons in culture and in brain tissue specimens in our experiment is not in contrast with the relevant physiologic role of estrogens on nervous central system, but it could be correlated to the embryonic period of life and could represent a protection of male brain from an undue estrogens imprinting. The mitomycin C, alkylation agent, has shown in our experiment a major neurotoxic and cytostatic power in comparison with methotrexate. Our conclusion is that human embryo neuronal culture in vitro is a powerful instrument for physiology and human therapy for cancer and neurodegenerative diseases.
Highlights
Histochemical confirmation of cellular neuronal phenotype was based on histochemical evidence of NSE (Neuron Specific Enolase).The detection of ERα in neuronal cells was performed with a rabbit Monoclonal Antibody
That is the culture of well differentiated neurons and not stem cells, from human embryo brain, is important for the research on hormones, drugs, neuromediators receptors with the aim to understand their role in morphogenesis and a potential therapeutic use or neurotoxicity
An original contribution of our paper is the study of estrogens receptor ERα on neurons in culture and toxicity test for Mitomycin C and Methotrexate on neurons in vitro
Summary
Neural cells culture has been successfully accomplished from neural precursors from marine embryonic stem cell [1] and from human ones [2]. The aim of the above mentioned researches was to obtain neural progenitors fit to integrate in vivo into a host tissue in order to replace damaged or absent neurons [3,4,5,6]. That is the culture of well differentiated neurons and not stem cells, from human embryo brain, is important for the research on hormones, drugs, neuromediators receptors with the aim to understand their role in morphogenesis and a potential therapeutic use or neurotoxicity. An original contribution of our paper is the study of estrogens receptor ERα on neurons in culture and toxicity test for Mitomycin C and Methotrexate on neurons in vitro
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