Human CD154 induces activation of porcine endothelial cells and up-regulation of MHC class II expression.

Abstract

CD40 is expressed on a number of antigen-presenting cells and also on vascular endothelium. It has been shown that engagement of CD40 on vascular endothelium by CD154 on platelets and CD154-bearing cell lines leads to the induction of adhesion molecule expression. Having cloned porcine CD40, and shown that it is capable of binding human CD154, we investigate whether human CD154 can activate porcine endothelial cells (EC) through CD40 ligation. Human Jurkat clone D1.1 (CD154+), or clone E6.1 (CD154-), were co-cultured with EC from pig aorta and human aorta and umbilical vein for various times in the presence or absence of blocking antibody to CD154. Human and pig EC were shown to express CD40 by flow cytometry by using soluble human CD154 (CD154Ckappa). Co-culture of pig EC with CD154-expressing Jurkat D1.1 cells led to the induction of E-selectin by 6 hr (peak 24 hr) and vascular cell adhesion molecule-1 (VCAM-1) by 6 hr (peak 48 hr). Similar results were also observed with human EC. Porcine EC were induced to up-regulate major histocompatibility complex class II at 24 hr by co-culture with Jurkat D1.1 cells through a CD40-dependent mechanism. In contrast, no up-regulation was observed on human EC. A number of cells can express CD154, including T cells, natural killer cells, and platelets, and these could signal graft EC through the CD40 pathway. These results demonstrate a possible role for the CD40 pathway in the activation of vascular endothelium in the rejection of porcine xenografts.