Abstract
Human bronchoalveolar macrophages were separated from other free lung cells by density sedimentation on Percoll gradients. They were then tested for cytotoxicity against the human lung adenocarcinoma cell line A549, using a Selenomethionine-75 post-labelling assay. The cytotoxicity of the macrophages increased as the effector:target cell ratio was increased, approaching 100% at 20:1. There was no significant difference in the cytotoxicity of macrophages isolated from the lungs of bronchial-carcinoma or non-carcinoma patients. The highly cytotoxic nature of the macrophages was not due to selection of a more potent cytotoxic subpopulation of macrophages on the Percoll gradient, nor to a generally elevated activation of the macrophages due to the pathological conditions in the patients' lungs. An attempt to determine whether low concentrations of macrophages could potentiate target-cell growth proved negative. Cytotoxicity of macrophages for cultured lung target cells was not restricted to A549 cells and is not in accordance with the view that defective bronchoalveolar macrophage cytotoxicity contributes to the emergence of bronchial neoplasia.
Highlights
Summary.-Human bronchoalveolar macrophages were separated from other free lung cells by density sedimentation on Percoll gradients
The yield of viable free lung cells obtained by bronchoalveolar lavage was very variable (1-3-42.3 x 106 total) as was the proportion of the major free lung cell types: Bronchoalveolar macrophages (BAM), lymphocytes and polymorphonuclear leucocytes (PMNL) (Table)
The aim of this study was to examine a potential role for human BAM in the host response to bronchial neoplasia
Summary
Summary.-Human bronchoalveolar macrophages were separated from other free lung cells by density sedimentation on Percoll gradients. They were tested for cytotoxicity against the human lung adenocarcinoma cell line A549, using a Selenomethionine-75 post-labelling assay. Since human macrophages are preferentially cytostatic for human target cells (Hogg & Balkwill, 1981), we used a cultured human lung adenocarcinoma cell line (A549) as targets This choice was determined by the fact that targets derived from histologically distinctive tissues may vary greatly in their susceptibility to cell-mediated cytotoxic attack (Lohmann-Matthes et at., 1978; Roder et al, 1979)
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