Hsps expression in three human glioma cell lines after radio-frequency field exposure

Abstract

AIM To investigate and compare the effect of ra-dio-frequency field exposure on stress response genes (Hsps) expression in three human glioma cell lines. METHODS Glioma cells were from MO54, A172 and T98 three human glioma cell lines. Exponentially growing cells were exposed to sham conditions or 1950 MHz continuous-wave for 1h. PC randomly determined which of the two waveguides was ex-posed. Specific absorption rates (SARs) were 1 and 10 W/kg. After treatment, the expression level of Hsp27 and p-Hs27 as well as Hsp70, was determined by Western blotting. RESULTS The Hsp27 expression level of A172, MO54 and T98 cells ex-posed to RF fields (SAR: 1 W/kg, 10 W/kg) for 1h showed no difference compared with sham-exposed cells. Phosphorylated Hsp27 level of A172, MO54 and T98 cells exposed to RF fields (SAR: 1 W/kg) for 1 h showed no differences compared with sham-exposed cells. However, exposure to an RF field at 10 W/kg for 1h slightly decreased the level of phosphorylated Hsp27 in MO54 cells other in A172 and T98 cells. For the expression of Hsp70 in A172, MO54 and T98 cells after expo-sure to RF fields (SAR: 1 W/kg and 10 W/kg) for 1 h, no obvi-ous changes in Hsp70 expression were observed in RF-exposed cells compared with sham-exposed cells. CONCLUSIONS Our results suggest 1950 MHz RF field has little or no apparent effect on HSP70 and HSP27 expression in MO54, A172 and T98 cells, it may only slightly inhibit the phosphorylation of Hsp27 at Serine 78 in MO54 cells.KeywordsRadio-frequency fieldHsp70Hsp27protein expressionglioma cells