Abstract
BackgroundThe purpose of the present study was to examine the in vitro responses of ERM cells under the combination of centrifugal and compression forces, in terms of their expression of HSP70 mRNA.MethodsThe ERM cells were positive for CK19 indicating that they were derived from the odontogenic epithelium. Cultured ERM cells were applied centrifugal force and compressing force at one to three times as mechanical forces. After addition of forces, cells were observed using scanning electron microscope (SEM) and were measured expression of HSP70 mRNA by RT-PCR.ResultsSEM observations showed the cells were flattened immediately after the application of mechanical force, but nuclear protrusions recovered the same as the control 3 h later. A significantly higher expression of HSP70 mRNA was observed in ERM cells under mechanical force compared with the control, but it gradually decreased with time. No accumulation of HSP70 mRNA expression occurred with intermittent force. However, the expression of HSP70 mRNA with intermittent force repeated 3 times was significantly higher compared with intermittent force applied only once or twice.ConclusionsThese findings suggest that ERM cells express HSP70 mRNA in response to mechanical force, and that intermittent force maintains the level of HSP70 mRNA expression.
Highlights
The purpose of the present study was to examine the in vitro responses of epithelial rest of Malassez (ERM) cells under the combination of centrifugal and compression forces, in terms of their expression of HSP70 mRNA
scanning electron microscope (SEM) observations The ERM cells were spread out and nucleus protrusions and flattened cytoplasm were evident in the control group
The height of the ERM cells was 0.78 ± 0.18 μm immediately after the centrifugation and it recovered to the control level at 3 h after the centrifugation
Summary
The purpose of the present study was to examine the in vitro responses of ERM cells under the combination of centrifugal and compression forces, in terms of their expression of HSP70 mRNA. Yamashiro et al reported that denervation of the inferior alveolar nerve leads to a reduced distribution of the ERM at 1 week and dento-alveolar ankylosis occurs at 6 weeks. They confirmed that ERM receptor was immune-positive for trkA, which shows a high-affinity to the NGF receptor and they concluded that the sensory nerve might play a regulatory role in maintaining the ERM [6]. Mine et al observed the healing process of the PDL after tooth re-plantation in rats and compared the occluded group with the nonoccluded group They found that dento-alveolar ankylosis was clearly detected in the non-occluded group, but was not detected in the occluded group. Only a few studies have been reported about changes of the ERM under the various kinds of mechanical forces in vitro [8]
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