Abstract
BackgroundCircular RNA (circRNA) has been demonstrated to participate in cervical cancer development. In this study, we analyzed the role of hsa_circ_0000520 in cervical cancer.MethodsFifty-two pairs of cervical cancer and adjacent normal tissue samples were collected, and five human cervical cancer cell lines were obtained followed by the detection of hsa_circ_0000520 expression. Nuclear-cytoplasmic isolation and fluorescence in situ hybridization were performed to analyze the subcellular localization of hsa_circ_0000520 while linear RNA was digested by RNase R. Gain- or loss-of function experiments on hsa_circ_0000520 were performed, followed by detection of cell proliferation and cell cycle by EdU, Cell Counting Kit-8, colony formation assay, and flow cytometry respectively.ResultsHsa_circ_0000520 and cyclin-dependent kinase 2 (CDK2) were highly expressed in cervical cancer tissues. Binding sites between microRNA-1296 (miR-1296) and hsa_circ_0000520 or CDK2 were verified. Antibody to Argonaute 2 (Ago2) could precipitate hsa_circ_0000520, indicating that hsa_circ_0000520 could competitively bind to miR-1296 via Ago2. Silencing hsa_circ_0000520 inhibited cervical cancer cell proliferation and promoted the inhibitory effects of miR-1296 on CDK2, thereby blocking cell cycle progression and promoting apoptosis.ConclusionThese results support the premise that targeting hsa_circ_0000520 can be a potential approach to combat cervical cancer.
Highlights
IntroductionCircular RNA (circRNA) has been demonstrated to participate in cervical cancer development
Circular RNA has been demonstrated to participate in cervical cancer development
To verify the results of bioinformatics analysis, reverse transcription quantitative polymerase chain reaction (RT-qPCR) was performed on 52 pairs of cervical cancer tissues and adjacent normal tissues collected from clinical specimens, showing that hsa_circ_0000520
Summary
Circular RNA (circRNA) has been demonstrated to participate in cervical cancer development. We analyzed the role of hsa_circ_0000520 in cervical cancer. The pathogenesis of cervical cancer has been extensively explored, In previous studies, circRNAs have been reported as biomarkers for cervical cancer. How hsa_circ_0000520 functions in cervical cancer is still under-studied. Ours is the first study investigating the correlation of hsa_circ_0000520 with cervical cancer. Hsa_circ_0005576 was found to regulate the expression of miR-153 in cervical cancer cells [10]. As for cyclindependent kinase 2 (CDK2), increased expression of CDK2 has been identified in the cervical cancer cell line HeLa as previously reported [12]. Our study explored whether hsa_circ_0000520 can exert certain functions affecting the progression of cervical cancer in association with miR-1296 and CDK2, with intent to identify novel targets for cervical cancer treatment
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