Abstract
A simple, rapid, precise, and accurate high-performance thin-layer chromatographic (HPTLC) method has been established and validated for simultaneous analysis of escitalopram oxalate (ESC) and clonazepam (CLO) in pharmaceutical preparations. Separation was achieved on a aluminum HPTLC plates coated with 0.2 mm layers of silica gel 60 F 254 with methanol-toluene-triethylamine 1:3.5:0.1 ( v/v ) as mobile phase. Densitometric quantification was performed at 253 nm by reflectance scanning. The R F values of ESC and CLO were 0.36 and 0.49, respectively. The linearity of the method was investigated in the range 50–150 μg mL −1 for ESC and 5–15 μg mL −1 for CLO. Percentage recoveries for ESC and CLO were 100.41 and 99.54%, respectively, by height and 100.21% and 99.99% by area. The method was validated for precision, accuracy, specificity, and ruggedness.
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