HPTLC FINGER PRINT AND HEPATOPROTECTIVE ACTIVITY OF VASAKASAVA AGAINST PARACETAMOL INDUCED HEPATOTOXICITY

Abstract

The present study was undertaken to evaluate the hepatoprotective activity of prepared Vasakasava in paracetamol intoxicated rats . Vasakasava was prepared by fermenting the decoction of Adhatoda vasica leaves using a solution of jaggery, flowers of Woodfordia fructicosa ( family : Lythraceae ) , and other powdered ingredients with subsequent stirring in a jar . After placing seven layers of mud smeared cloth, the jar was kept undisturbed . Further, HPTL C was performed to determine the amount of vasicine in Vasakasava . The antihepatotoxic effect of Vasakasava ( 0 . 45 and 0 . 90ml / 250g p . o .) was tested against paracetamol induced liver toxicity ( 2g / kg p . o .). After 7 days of respective treatment, animals were sacrificed, MDA and reduced GSH were evaluated for oxidative stress, hepatic serum enzymes and histopathology was performed to investigate the de gree of hepatic injury . HPTLC analysis revealed the presence of 0 . 032 % vasicine in the formulation . Pre - adminis tration of Vasakasava restored the levels of SGOT, SGPT, and ALP to 77 . 85, 78 . 97, and 68 . 27 IU / L, respectively ( at 0 . 90ml / 250g p . o .) and 81 . 1, 153 . 4 and 87 . 66 IU / L respectively ( at 0 . 45ml / 250g p . o .). Similarly, the level of total bilirubin and direct bilir ubin was also normalized . The hepatoprotective effect was supported by histopathological examination of liver tissue . As a conclusion, Vasakasava possesses the potent hepatoprotective effect against paracetamol induced liver damage .