Abstract
A simple, rapid and precise HPLC method has been developed for the assay of hydrochlorothiazide in urine. The clean-up of the urine samples was carried out by solid-phase extraction using HLB cartridges. Extraction recovery was 94.00-100.28 %. HPLC separation was performed with isocratic elution on Hypersil BDS C18 column (100 x 4.0 mm I.D., 3 µm particle size) protected with appropriate guard column. The mobile phase was 18 % acetonitrile and 0.025 mol/L solution of KH2PO4, pH 4 at flow rate of 0.3 mL/min. Detection of the substances was performed at 220 nm. The calibration curves were linear in the range of 2-50 µg/mL. The developed method is validated by checking its accuracy, precision and stability. The detection limit is 2 µg/mL hydrochlorothiazide. The method is proved to be convenient for routine analysis of hydrochlorothiazide in urine.
Highlights
Hydrochlorothiazide is a thiazide diuretic drug (Fig. 1), which is used in treatment of hypertension, either alone or together with other agents, it is effective in treatment of congestive heart failure and hepatic disorders
Several investigations have been performed for determination of hydrochlorothiazide in urine by high-performance liquid chromatography (HPLC) with UV detection and electrochemical detection
The optimization of the HPLC conditions was performed for hydrochlorothiazide and cefotaxime, which was tested as an internal standard
Summary
Hydrochlorothiazide is a thiazide diuretic drug (Fig. 1), which is used in treatment of hypertension, either alone or together with other agents, it is effective in treatment of congestive heart failure and hepatic disorders. In medical practice, it is mostly used in a conventional tablet form in dose of 25 to 250 mg [1]. Domingo and coworkers [6] used micellar liquid chromatography for determination of hydrochlorothiazide in urine by direct injection of sample in the chromatographic system without any sample pretreatment. Papadoyannis and coworkers [8, 9] have performed a chromatographic deter-
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