Abstract
The products of γ-irradiation of tripeptides (AWA, LWL, LWM) and lysozyme were determined by HPLC and UV/fluorescence detection. A fast and simple one-step hydrolysis with pronase E (30 – 60 min, 40°C) was developed to release the radiation products, without damage, from the peptide chain. N-Formylkynurenine (NFK), oxindolylalanine (OIA), 4-, 5-, 6- and 7-hydroxytryptophan were the main products of irradiation of peptides and lysozyme. It is possible that the nonphysiological hydroxytryptophan isomers 4-, 6- and 7-hydroxytryptophan could serve as marker substances for irradiated food with a high protein content.
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