How does the unicellular Tetrahymena utilise the hormones that it produces? Paying a visit to the realm of atto-and zeptomolar concentrations

Abstract

Changes in the hormone content of Tetrahymena pyriformis GL were investigated during histamine, serotonin or insulin treatment at concentrations of 10(-6)M to 10(-21)M for 30 min. The immunologically demonstrable hormone content was studied by using specific antibodies, flow cytometry and confocal microscopy. Histamine at the higher ranges elevated the serotonin content of Tetrahymena, whereas serotonin at the lower ranges (down to 10(-21)M) decreased its histamine levels. Insulin did not affect its serotonin content, whereas serotonin increased its insulin content at each concentration studied (down to 10(-21)M). Insulin between 10(-6)M and 10(-21)M increased the histamine levels of Tetrahymena, although histamine influenced its insulin level only at 10(-6)M. Our results call attention to the presence of hormonal interactions even at "low" levels of phylogeny and to the extreme sensitivity of the hormone receptors of Tetrahymena. These data might explain (1) the requirement of Tetrahymena for (vertebrate) hormone production and hormone receptors and (2) the way that it uses these hormones under natural conditions.