Abstract
BackgroundBrucellosis is a worldwide zoonotic infectious disease that is transmitted in various ways and causes great harm to humans and animals. The brucellosis pathogen is Brucella, which mainly resides in macrophage cells and survives and replicates in host cells. However, the mechanisms underlying Brucella survival in macrophage cells have not been thoroughly elucidated to date. Peroxiredoxin 6 (Prdx6) is a bifunctional protein that shows not only GSH peroxidase activity but also phospholipase A2 activity and plays important roles in combating oxidative damage and regulating apoptosis.ResultsRecombinant mouse (Mus musculus) Prdx6 (MmPrdx6) was expressed and purified, and monoclonal antibodies against MmPrdx6 were prepared. Using the Brucella suis S2 strain to infect RAW264.7 murine macrophages, the level of intracellular Prdx6 expression first decreased and later increased following infection. Overexpressing Prdx6 in macrophages resulted in an increase in B. suis S2 strain levels in RAW264.7 cells, while knocking down Prdx6 reduced the S2 levels in cells.ConclusionsHost Prdx6 can increase the intracellular survival of B. suis S2 strain and plays a role in Brucella infection.
Highlights
Brucellosis is a worldwide zoonotic infectious disease that is transmitted in various ways and causes great harm to humans and animals
Positive clones were screened through bacterial PCR, and the integrity of the plasmids was verified by DNA sequencing, revealing 100% sequence identity to the Peroxiredoxin 6 (Prdx6) gene
The results of Sodium Dodecyl Sulfonate (SDS)-PAGE showed an approximately 28 kDa band in the induced cells, indicating that the recombinant Prdx6 protein was successfully expressed in the transformed E. coli BL21(DE3) cells compared to uninduced cells (Fig. 1a)
Summary
Brucellosis is a worldwide zoonotic infectious disease that is transmitted in various ways and causes great harm to humans and animals. The brucellosis pathogen is Brucella, which mainly resides in macrophage cells and survives and replicates in host cells. Peroxiredoxin 6 (Prdx6) is a bifunctional protein that shows GSH peroxidase activity and phospholipase A2 activity and plays important roles in combating oxidative damage and regulating apoptosis. The intracellular pathogen of Brucella spp. can cause brucellosis [1], which is one of the most important zoonoses worldwide, especially in developing countries, where epidemics may be severe. Survival and replication properties of Brucella in host cells contribute to its virulence [3]. Prdx, which shows GSH peroxidase (NSGPx) activity and phospholipase A2 (PLA2) activity [8] is the only 1-Cys member of the peroxiredoxin superfamily in mammalian [9].
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